РЕЗУЛЬТАТЫ БИОХИМИЧЕСКИХ ИССЛЕДОВАНИЙ КУЛЬТУР, ВЫДЕЛЕННЫХ В КОНТРОЛЬНЫХ ТОЧКАХ ОБЪЕКТОВ ПРИ ПРОИЗВОДСТВЕ ГОВЯДИНЫ В ТОО “БАЙСЕРКЕ-АГРО” АЛМАТИНСКОЙ ОБЛАСТИ
DOI:
https://doi.org/10.52578/2305-9397-2024-3-1-237-246Ключевые слова:
Хозяйство, убойный пункт, транспорт, биохимические показатели, изолят, углеводы, среда Гисса, агар КристенсенаАннотация
Целью наших исследований явилось изучение биохимических свойств микроорганизмов, выделенных в контрольных точках при производстве говядины в условиях ТОО «Байсерке-Агро» Алматинской области.
Исследования были проведены в ТОО "Байсерке Агро", расположенным в сельском округе Панфилово Талгарского района Алматинской области и в учебно-научной лаборатории “Серология” имени академика НАН РК, доктора ветеринарных наук, профессора Т.Сайдулдина при кафедре биологической безопасности КазНАИУ.
Взяли пробы из трех объектов (хозяйство, транспорт, убойный пункт) в нескольких контрольных точках.
Все пробы были исследованы бактериологически и обнаружены культуры в хозяйстве из трех точек: передний край автопоилки, передний край кормушки и боковые стороны стойки; из кузова прицепа – из пола; в убойном пункте - в убойном цехе – из пола станка, крючка для подвешивания туш, ножа для снятия шкуры. Итого из 7 точек были выделены культур.
Эти изоляты прошли биохимические исследования. Для этого использовали стандартную среду Гисса с различными углевадами такими как лактоза, сахароза, мальтоза, маннит, сорбит и агар Кристенсена.
Был проведен кластерный анализ (по возрастающей иерархической классификации -AHC) по методу Уорда для идентификации групп бактерий. Для оценки связи между группами и местом отбора проб была составлена таблица сопряженности.
Изоляты №№ 1, 3, 4, 6 и 7 не расщепляли лактозу, мальтозу, сахарозу, маннит, глюкозу и сорбит, и не дали рост в среде Кристенсена. Тогда как изолят №2 расщеплял все углеводы, а изолят №5 – расщеплял сахарозу. В среде Кристенсена хорошо росли 2 и 5 культуры.
Таблица сопряжённости не нашла связи между группами бактерии и местом отбора бактерии (p value = 0.891).
Библиографические ссылки
WHO Estimates of the Global Burden of Foodborne Diseases: Foodborne Disease Burden Epidemiology Reference Group 2007–2015, [Text] World Health Organization, Geneva, Switzerland, 2015.
Mangen, M. J., Disease Burden of Food-Related Pathogens in the Netherlands, RIVM, Bilthoven, Netherlands [Text] / M. J. Mangen [and etc.] // 2018-80, doi:10.21945/RIVM-2020117.
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Antic, D. Distribution of microflora on cattle hides and its transmission to meat via direct contact. [Text] / D.Antic [and etc.] // Food Control. – 2010. – Vol. 21. – P.1025–1029.
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Sudhakar G. Bacteriological screening of environmental sources of contaminationin an abattoir and the meat shops in Mumbai [Text] / G. Sudhakar [and etc.] // J. Food Ag-Ind. – 2009. –
Vol. 2(3). – P.277-287.
WHO, Food Safety and Food Borne Illness [Text] / Fact sheet No. 237, World Health Organization, Geneva, Switzerland, 2007.
Gill, C.O. Microbiological contamination of meat during slaughter and butchering of cattle, sheep and pigs [Text] / In: Davies, A.; Board, R. (Eds.) // The Microbiology of Meat and Poultry. London: Blackie Academic and Professional. – 1998. - P. 118-157.
Abdou E.A. Application of food safety in developing countries. Epidemiology of meatborne and milkborne infections in the Mediterranean region [Text] / E.A. Abdou // Inf. Circ. WHO/Mediter. Zoon. Control Centre. – 2002. – Vol.54. – P.6-9.
U.S. Department of Agriculture. USDA-APHIS: EHEC O157 0157 and Salmonella-Status on U.S. dairy operations. [Text] / Centers for Epidemiology and Animal Health. Fort Collins, Colo. - 1996.
Nørrung, B. Microbial safety of meat in the European Union [Text] / B. Nørrung [and etc.] // Meat Science. – 2008. – Vol. 78(1–2). – P. 14–24.
Nortjé, G. L. A microbiological survey of fresh meat in the supermarket trade. Part 1: Carcasses and contact surfaces [Text] / G. L. Nortjé [and etc.] // Meat Science. - 1989a. – Vol. 25(2). – P. 81–97.
Everitt, B.S. Cluster analysis (4th edition) [Text] / B.S. Everitt [and etc.] // Arnold, London. -2001. – 237 p.
REFERENCES
WHO Estimates of the Global Burden of Foodborne Diseases: Foodborne Disease Burden Epidemiology Reference Group 2007–2015, [Text] World Health Organization, Geneva, Switzerland, 2015.
Mangen, M. J., Disease Burden of Food-Related Pathogens in the Netherlands, RIVM, Bilthoven, Netherlands [Text] / M. J. Mangen [and etc.] // 2018-80, doi:10.21945/RIVM-2020117.
Choi, Y.M. Application of supercritical carbon dioxide for microorganism reductions in fresh pork.Bae [Text] Y.M. Choi [and etc.] // Journal of Food Safety/ - 2011. – Vol.31/ - P.511–517.
Bae, Y.Y. Effects of supercritical carbon dioxide treatment against generic Escherichia coli, Listeria monocytogenes, Salmonella typhimurium, and E. coli O157: H7 in marinades and marinated pork Choi [Text] / Bae, Y.Y. [and etc.] // Meat Science. - 2009. – Vol. 82. – P.419–424.
Gill, C.O. Microbiological conditions of meats from large game animals and birds. [Text] / Gill, C.O. // Meat Science. – 2007. – Vol. 77. – P.149–160.
Nouichi, S. Superficial bacterial contamination of ovine and bovine carcasses at El-Harrach Slaughterhouse (Algeria) [Text] / S. Nouichi [and etc.] // European Journal of Scientific Research. – 2009. – Vol. 38(3). – P.474-485.
Okonko I. Assessment of bacteriological quality of fresh Meats sold in Calabar Metropolis [Text] / I. Okonko [and etc.] // Electronic Journal of Environmental, Agricultural and Food Chemistry. – 2010. - Vol. 9. - No. 1. - P. 89–100.
Nouichi, S. Distribution of Salmonella virulence factors originated from sheep and cattle in Algerian slaughterhouses. [Text] / S. Nouichi [and etc.] // Journal of the Hellenic Veterinary Medical Society. – 2023. – Vol.73(4). – P.5013–5020. https://doi.org/10.12681/jhvms.29148.
Antic, D. Distribution of microflora on cattle hides and its transmission to meat via direct contact. [Text] / D.Antic [and etc.] // Food Control. – 2010. – Vol. 21. – P.1025–1029.
The European Union summary report on trends and sources of zoonotic agents and food-borne outbreaks in 2010. [Text] / EFSA Journal. 2012b.- Vol. 10(3). – P. 2597 (442 pp.).
Scientific opinion on the public health hazards to be covered by inspection of meat (poultry). [Text] / EFSA Journal. - 2012a. – P.2741.
Adugna, M. Pal Prevalence and antibiogram assessment of Staphylococcus aureus in beef at municipal abattoir and butcher shops in Addis Ababa, Ethiopia [Text] / M. Pal Adugna [and etc.] // BioMed Research International. – 2018. - Article ID 5017685. - 7 p. doi:10.1155/2018/5017685.
Birhanu, S. Assesment of microbiological quality and meat handling practices in butcher shops and abattoir found in Gondar town, Ethiopia [Text] / S. Birhanu [and etc.] // International Journal of Microbiological Research. – 2017. - Vol. 8. - No. 2. - P. 59–68.
Cooper, K. The Plant Environment Counts: Protect your Product through Environmental Sampling. [Text] / K. Cooper // Meat and Poultry. – 1999. – Vol. – 5. – P.5.
Sofos J.N. Sources and extent of microbiological contamination of beef carcasses in seven United States slaughtering plants. [Text] / J.N. Sofos [and etc.] // J. Food Prot. – 1999. – Vol. 62(2). – P. 140-5.
Sudhakar G. Bacteriological screening of environmental sources of contaminationin an abattoir and the meat shops in Mumbai [Text] / G. Sudhakar [and etc.] // J. Food Ag-Ind. – 2009. –
Vol. 2(3). – P.277-287.
WHO, Food Safety and Food Borne Illness [Text] / Fact sheet No. 237, World Health Organization, Geneva, Switzerland, 2007.
Gill, C.O. Microbiological contamination of meat during slaughter and butchering of cattle, sheep and pigs [Text] / In: Davies, A.; Board, R. (Eds.) // The Microbiology of Meat and Poultry. London: Blackie Academic and Professional. – 1998. - P. 118-157.
Abdou E.A. Application of food safety in developing countries. Epidemiology of meatborne and milkborne infections in the Mediterranean region [Text] / E.A. Abdou // Inf. Circ. WHO/Mediter. Zoon. Control Centre. – 2002. – Vol.54. – P.6-9.
U.S. Department of Agriculture. USDA-APHIS: EHEC O157 0157 and Salmonella-Status on U.S. dairy operations. [Text] / Centers for Epidemiology and Animal Health. Fort Collins, Colo. - 1996.
Nørrung, B. Microbial safety of meat in the European Union [Text] / B. Nørrung [and etc.] // Meat Science. – 2008. – Vol. 78(1–2). – P. 14–24.
Nortjé, G. L. A microbiological survey of fresh meat in the supermarket trade. Part 1: Carcasses and contact surfaces [Text] / G. L. Nortjé [and etc.] // Meat Science. - 1989a. – Vol. 25(2). – P. 81–97.
Everitt, B.S. Cluster analysis (4th edition) [Text] / B.S. Everitt [and etc.] // Arnold, London. -2001. – 237 p.
