СИЫР ЭНДОМЕТРИТІ АУРУЫНА СЕБЕПШІ МИКРООРГАНИЗМДЕРДІҢ ВИРУЛЕНТТІЛІГІ МЕН АНТИБИОТИККЕ РЕЗИСТЕНТТІЛІГІН АНЫҚТАУ
СИЫР ЭНДОМЕТРИТІ АУРУЫНА СЕБЕПШІ МИКРООРГАНИЗМДЕРДІҢ ВИРУЛЕНТТІЛІГІ МЕН АНТИБИОТИККЕ РЕЗИСТЕНТТІЛІГІН АНЫҚТАУ
DOI:
https://doi.org/10.52578/2305-9397-2025-2-2-76-88%20Ключевые слова:
Эндометрит, сиырлар, Escherichia coli, Staphylococcus aureus, fimH гені, Nuc гені, ПЦР, антибиотикке резистенттілік, вируленттілік, репродуктивті патология.Аннотация
Эндометрит – Қазақстаның сиыр шаруашылықтарында жиі кездесетін репродуктивті
патологиялық аурулардың бірі. Бұл зерттеудің мақсаты – туғаннан кейінгі сиырлардағы
эндометрит қоздырғыштарының патогендігін анықтау және тиімді емдеу шараларын жүргізу үшін
олардың антибиотикке резистенттілігін зерттеу.
76
Зерттеу Абай облысындағы шаруашылықта ұсталатын голштейн тұқымды сауын
сиырларында (n=100) жүргізілді. Жатырдан тупфер арқылы алынған патологиялық материалдан
жағындылар дайындалып, ЕПА ортасында өсірілді және ПЦР классикалық әдісі арқылы зерттелді.
Қоздырғыш ретінде табылған Escherichia coli (fimH гені) және Staphylococcus aureus (Nuc гені)
арнайы праймерлерді қолдану арқылы расталды. Қоздырғыштардың таза өсінділеріне дискті
диффузды әдіспен шаруашылықта қолданылып жүрген сегіз түрлі антибиотиктің әсері зерттелді.
Зерттеуге алынған 100 сиырдың 21-інен эндометрит анықталды. Оның ішінде 11 үлгіден
Escherichia coli, ал 10 үлгіден Staphylococcus aureus қоздырғыштары бөлініп алынды.
Антибиотикке резистенттілікті анықтау барысында қолданылған сегіз антибиотиктің екеуі тиімсіз
болып танылды.
Эндометрит қоздырғыштарының антибиотикке төзімділігін зерттеу емдеу шараларын дұрыс
жоспарлауға мүмкіндік береді. Патогендік микроорганизмдердің генетикалық детерминанттарын
анықтау үшін ПЦР әдісінің қолданылуы диагностиканың дәлдігін арттырып, қоздырғыштарды
жедел әрі сенімді түрде анықтауға мүмкіндік берді. Бұл зерттеу шаруашылық жағдайында
эндометритті тиімді емдеу үшін қолданылатын антибиотиктерді таңдауда және аурудың алдын алу
шараларын жақсартуда ғылыми негіз бола алады.
Библиографические ссылки
ӘДЕБИЕТТЕР ТІЗІМІ
Dubuc, J. Definitions and diagnosis of postpartum endometritis in dairy cows [Text] / J. Dubuc
[and etc.] // Journal of Dairy Science. 2010. – Vol. 93. – № 11. – P. 5225–5233.
Wagener, K. A review of the ongoing discussion about definition, diagnosis, and
pathomechanism of subclinical endometritis in dairy cows [Text] / K. Wagener [and etc.] //
Theriogenology. 2017. – Vol. 94. – P. 21–30.
Westermann, S. A clinical approach to determine false positive findings of clinical endometritis
by vaginoscopy by the use of uterine bacteriology and cytology in dairy cows [Text] /
S. Westermann [and etc.] // Theriogenology. 2010. – Vol. 74. – № 7. – P. 1248–1255.
Jakupov, I. The effectiveness of uterine disease diagnosis methods in cows depending on
postpartum days [Text] / 4
I. Jakupov [and etc.] // KAZATUVC. 2023. – № 3(003). – P. 68–74.
Husnain, A. Induced endometritis in early lactation compromises production and reproduction
in dairy cows [Text] / A. Husnain [and etc.] // Journal of Dairy Science. 2023. – Vol. 106. – № 6. – P.
–4213.
Mohammed, Z.A. Impact of endometritis on post-partum ovarian cyclicity in dairy cows [Text]
/ Z.A. Mohammed [and etc.] // The Veterinary Journal. 2019. – Vol. 248. – P. 8–13.
Paiano, R.B. Evaluation of clinical and subclinical endometritis impacts on the reproductive
performance and milk production of dairy cows in Brazilian herds [Text] / R.B. Paiano [and etc.] //
Reprod Domestic Animals. 2023. – Vol. 58. – № 3. – P. 414–422.
Boni, R. Relationship between oxidative stress and endometritis: exploiting knowledge gained
in mares and cows [Text] / R. Boni [and etc.] // Animals. 2022. – Vol. 12. – № 18. – P. 2403.
Bretzlaff, K. Rationale for treatment of endometritis in the dairy cow [Text] / K. Bretzlaff //
Veterinary Clinics of North America: Food Animal Practice. 1987. – Vol. 3. – № 3. – P. 593–607.
LeBlanc, S.J. Defining and diagnosing postpartum clinical endometritis and its impact on
reproductive performance in dairy cows [Text] / S.J. LeBlanc [and etc.] // Journal of Dairy Science. 2002. – Vol. 85. – № 9. – P. 2223–2236.
Nyabinwa, P. Estimating prevalence of endometritis in smallholder zero-grazed dairy cows in
Rwanda [Text] / P. Nyabinwa [and etc.] // Trop Anim Health Prod. 2020. – Vol. 52. – № 6. –
P. 3135–3145.
Gonzalez Moreno, C. Characterization of native Escherichia coli populations from bovine
vagina of healthy heifers and cows with postpartum uterine disease [Text] / C. Gonzalez Moreno [and
etc.] // PLoS ONE/ed. Butaye, P. 2020. – Vol. 15. – № 6. – P. e0228294.
Yamamura, F. Relationship between Escherichia coli virulence factors, notably kpsMTII, and
symptoms of clinical metritis and endometritis in dairy cows [Text] / F. Yamamura [and etc.] //
J. Vet. Med. Sci. 2022. – Vol. 84. – № 3. – P. 420–428.
Sheldon, I. Specific strains of Escherichia coli are pathogenic for the endometrium of cattle and
cause pelvic inflammatory disease in cattle and mice [Text] / I. Sheldon [and etc.] // PLoS ONE. 2010. –
Vol. 5.
Williams, E. Effect of Escherichia coli infection of the bovine uterus from the whole animal to
the cell [Text] / E. Williams [and etc.] // Animal. 2008. – Vol. 2. – № 8. – P. 1153–1157.
Shafique, L. Evidence-based tracking of MDR E. coli from bovine endometritis and its
elimination by effective novel therapeutics [Text] / L. Shafique [and etc.] // Antibiotics. 2021. –
Vol. 10. – № 8. – P. 997.
Raheel, I. Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis
and their antibiotic resistance profiles [Text] / I. Raheel [and etc.] // Journal of Advanced Veterinary and
Animal Research. 2020. – Vol. 7. – P. 442–451.
Becker, A. The endometrial microbiota—16S rRNA gene sequence signatures in healthy,
pregnant and endometritis dairy cows [Text] / A. Becker [and etc.] // Veterinary Sciences. 2023. –
Vol. 10. – № 3. – P. 215.
Husnain, A. Induced endometrial inflammation compromises conceptus development in dairy
cattle [Text] / A. Husnain [and etc.] // Biology of Reproduction. 2023. – Vol. 109. – № 4. –
P. 415–431.
Sheldon, I.M. Tolerance and innate immunity shape the development of postpartum uterine
disease and the impact of endometritis in dairy cattle [Text] / I.M. Sheldon [and etc.] // Annu. Rev. Anim.
Biosci. 2019. – Vol. 7. – № 1. – P. 361–384.
Wagener, K. A Review of the ongoing discussion about definition, diagnosis and
pathomechanism of subclinical endometritis in dairy cows [Text] / K. Wagener [and etc.] //
Theriogenology. 2017. – Vol. 94. – P. 21–30.
Sheldon, I.M. Specific strains of Escherichia coli are pathogenic for the endometrium of cattle
and cause pelvic inflammatory disease in cattle and mice [Text] / I.M. Sheldon [and etc.] // PLoS ONE /
ed. Sturtevant J. 2010. – Vol. 5. – № 2. – P. e9192.
Shafique, L. Evidence-based tracking of MDR E. coli from bovine endometritis and its
elimination by effective novel therapeutics [Text] / L. Shafique [and etc.] // Antibiotics. 2021. –
Vol. 10. – № 8. – P. 997.
Becker, A. The endometrial microbiota—16S rRNA gene sequence signatures in healthy,
pregnant and endometritis dairy cows [Text] / A. Becker [and etc.] // Veterinary Sciences. 2023. –
Vol. 10. – № 3. – P. 215.
Horiuk, Y. Characterization of lytic activity of Phage SAvB14 on Staphylococcus aureus
variant bovis [Text] / Y. Horiuk [and etc.] // Journal of Advanced Veterinary and Animal Research. 2020. – Vol. 7. – № 3. – P. 509.
Au, J. Correction: Role of autocrine bone morphogenetic protein signaling in trophoblast stem
cells [Text] / J. Au [and etc.] // Biology of Reproduction. – 2023. – Vol. 108. – № 6. – P. 997.
Yamasaki, E. Prevalence of Shiga toxin-producing Escherichia coli in Yezo sika deer (Cervus
nippon yesoensis) in the Tokachi sub-prefecture of Hokkaido, Japan [Text] / E. Yamasaki, [and etc.] // J.
Vet. Med. Sci. 2022. – Vol. 84. – № 6. – P. 770–776.
REFERENCES
Dubuc, J. Definitions and diagnosis of postpartum endometritis in dairy cows [Text] / J. Dubuc
[and etc.] // Journal of Dairy Science. 2010. – Vol. 93. – № 11. – P. 5225–5233.
Wagener, K. A review of the ongoing discussion about definition, diagnosis and
pathomechanism of subclinical endometritis in dairy cows [Text] / K. Wagener [and etc.] //
Theriogenology. 2017. – Vol. 94. – P. 21–30.
Westermann, S. A clinical approach to determine false positive findings of clinical endometritis
by vaginoscopy by the use of uterine bacteriology and cytology in dairy cows [Text] /
S. Westermann [and etc.] // Theriogenology. 2010. – Vol. 74. – № 7. – P. 1248–1255.
Jakupov, I. The effectiveness of uterine disease diagnosis methods in cows depending on
postpartum days [Text] / 4
I. Jakupov [and etc.] // KAZATUVC. 2023. – № 3(003). – P. 68–74.
Husnain, A. Induced endometritis in early lactation compromises production and reproduction
in dairy cows [Text] / A. Husnain [and etc.] // Journal of Dairy Science. 2023. – Vol. 106. – № 6. –P.
–4213.
Mohammed, Z.A. Impact of endometritis on post-partum ovarian cyclicity in dairy cows [Text]
/ Z.A. Mohammed [and etc.] // The Veterinary Journal. 2019. – Vol. 248. – P. 8–13.
Paiano, R.B. Evaluation of clinical and subclinical endometritis impacts on the reproductive
performance and milk production of dairy cows in Brazilian herds [Text] / R.B. Paiano [and etc.] //
Reprod Domestic Animals. 2023. – Vol. 58. – № 3. – P. 414–422.
Boni, R. Relationship between oxidative stress and endometritis: exploiting knowledge gained
in mares and cows [Text] / R. Boni [and etc.] // Animals. 2022. – Vol. 12. – № 18. – P. 2403.
Bretzlaff, K. Rationale for treatment of endometritis in the dairy cow [Text] / K. Bretzlaff //
Veterinary Clinics of North America: Food Animal Practice. 1987. – Vol. 3. – № 3. – P. 593–607.
LeBlanc, S.J. Defining and diagnosing postpartum clinical endometritis and its impact on
reproductive performance in dairy cows [Text] / S.J. LeBlanc [and etc.] // Journal of Dairy Science. 2002. – Vol. 85. – № 9. – P. 2223–2236.
Nyabinwa, P. Estimating prevalence of endometritis in smallholder zero-grazed dairy cows in
Rwanda [Text] / P. Nyabinwa [and etc.] // Trop Anim Health Prod. 2020. – Vol. 52. – № 6. –
P. 3135–3145.
Gonzalez Moreno, C. Characterization of native Escherichia coli populations from bovine
vagina of healthy heifers and cows with postpartum uterine disease [Text] / C. Gonzalez Moreno [and
etc.] // PLoS ONE / ed. Butaye P. 2020. – Vol. 15. – № 6. – P. e0228294.
Yamamura, F. Relationship between Escherichia coli virulence factors, notably kpsMTII, and
symptoms of clinical metritis and endometritis in dairy cows [Text] / F. Yamamura [and etc.] //
J. Vet. Med. Sci. 2022. – Vol. 84. – № 3. – P. 420–428.
Sheldon, I. Specific strains of Escherichia coli are pathogenic for the endometrium of cattle and
cause pelvic inflammatory disease in cattle and mice [Text] / I. Sheldon [and etc.] // PLoS ONE. 2010. –
Vol. 5.
Williams, E. Effect of Escherichia coli infection of the bovine uterus from the whole animal to
the cell [Text] / E. Williams [and etc.] // Animal. 2008. – Vol. 2. – № 8. – P. 1153–1157.
Shafique, L. Evidence-based tracking of MDR E. coli from bovine endometritis and its
elimination by effective novel therapeutics [Text] / L. Shafique [and etc.] // Antibiotics. 2021. –
Vol. 10. – № 8. – P. 997.
Raheel, I. Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis
and their antibiotic resistance profiles [Text] / I. Raheel [and etc.] // Journal of Advanced Veterinary and
Animal Research. 2020. – Vol. 7. – P. 442–451.
Becker, A. The endometrial microbiota—16S rRNA gene sequence signatures in healthy,
pregnant and endometritis dairy cows [Text] / A. Becker [and etc.] // Veterinary Sciences. 2023. – Vol.
– № 3. – P. 215.
Husnain, A. Induced endometrial inflammation compromises conceptus development in dairy
cattle [Text] / A. Husnain [and etc.] // Biology of Reproduction. 2023. – Vol. 109. – № 4. –
P. 415–431.
Sheldon, I.M. Tolerance and innate immunity shape the development of postpartum uterine
disease and the impact of endometritis in dairy cattle [Text] / I.M. Sheldon [and etc.] // Annu. Rev. Anim.
Biosci. 2019. – Vol. 7. – № 1. – P. 361–384.
Wagener, K. A review of the ongoing discussion about definition, diagnosis, and
pathomechanism of subclinical endometritis in dairy cows [Text] / K. Wagener [and etc.] //
Theriogenology. 2017. – Vol. 94. – P. 21–30.
Sheldon, I.M. Specific strains of Escherichia coli are pathogenic for the endometrium of cattle
and cause pelvic inflammatory disease in cattle and mice [Text] / I.M. Sheldon [and etc.] // PLoS ONE /
ed. Sturtevant, J. 2010. – Vol. 5. – № 2. – P. e9192.
Shafique, L. Evidence-based tracking of MDR E. coli from bovine endometritis and its
elimination by effective novel therapeutics [Text] / L. Shafique [and etc.] // Antibiotics. 2021. – Vol. 10. – № 8. – P. 997.
Becker, A. The endometrial microbiota—16S rRNA gene sequence signatures in healthy,
pregnant and endometritis dairy cows [Text] / A. Becker [and etc.] // Veterinary Sciences. 2023. – Vol.
– № 3. – P. 215.
Horiuk, Y. Characterization of lytic activity of Phage SAvB14 on Staphylococcus aureus
variant bovis [Text] / Y. Horiuk [and etc.] // Journal of Advanced Veterinary and Animal Research. 2020. – Vol. 7. – № 3. – P. 509.
Au, J. Correction: Role of autocrine bone morphogenetic protein signaling in trophoblast stem
cells [Text] / J. Au [and etc.] // Biology of Reproduction. – 2023. – Vol. 108. – № 6. – P. 997.
Yamasaki, E. Prevalence of Shiga toxin-producing Escherichia coli in Yezo sika deer (Cervus
nippon yesoensis) in the Tokachi sub-prefecture of Hokkaido, Japan [Text] / E. Yamasaki, [and etc.] // J.
Vet. Med.. Sci. 2022. – Vol. 84. – № 6. – P. 770–776.
