РЕЗУЛЬТАТЫ ГЕНОТИПИРОВАНИЯ КОРОВ ГОЛШТИНСКОЙ ПОРОДЫ ПО ЛОКУСАМ ГЕНОВ MX1, CXCR1 И СВЯЗЬ АЛЛЕЛЕЙ ЭТИХ ГЕНОВ С УСТОЙЧИВОСТЬЮ К МАСТИТУ У КОРОВ
ветеринария
DOI:
https://doi.org/10.52578/2305-9397-2025-1-1-3-14Ключевые слова:
голштинская порода, генетическая резистентность к маститам, ДНК маркеры, гены МХ1, CXCR1 c. 291C>T, CXCR1 1093C>T,, ПЦР-ПДРФ анализ, комбинированные генотипы,, желательный генотип.Аннотация
Авторами статьи проведено генотипирования коров голштинской породы по локусам генов: MX1 g.143182088, CXCR1 c.+291C>T, CXCR1 +1093C>T, ассоцированных с резистентностью к маститам у коров, выявление животных с желательным генотипом, с минимальным риском заболеваемости субклиническим маститом. По локусам генов MX1 c.567 T>C, CXCR1 c.+291C>T, CXCR1 +1093C>T наблюдается генетический полиморфизм, распространенность генетических вариантов была: MX1 - TT –0,12, CT – 0,30, CC – 0,58, CXCR1 c.+291C>T - TT - 0,12, CT – 0,33, CC – 0,55, CXCR1 +1093C>T - TT - 0,47, CT – 0,51, CC – 0,02. По двум локусам генов, MX1 g.143182088 и CXCR1 +1093C>T выявлен факт нарушения генного равновесия и вследствие чего, установлено увеличение значения χ2 до 10,6261 и 9,7137, соответственно. Методом ПЦР-ПДРФ анализа были идентифицированы комбинированные генотипы 40 коров голштинской породы ТОО «Амиран» и в течение года ежеквартально проводилась диагностика на субклинический мастит, у животных с комбинированным СССССТ генотипом по локусам генов MX1 c.567 T>C, CXCR1 c.+291C>T, CXCR1 +1093C>T обнаружен высокий риск заболеваемости субклиническим маститом. Таким образом, локусы генов MX1, CXCR1 являются потенциальными ДНК маркерами устойчивости к субклиническим маститам у коров голштинской породы.
Библиографические ссылки
ӘДЕБИЕТТЕР ТІЗІМІ
Karthikeyan, A. Genetic basis of mastitis resistance in cattle. International Journal of Science, [Text] / A. Karthikeyan //Environment ISSN 2278-3687 (O) and Technology, Vol. 5, No 4, 2016, 2192 – 2199
Muhammad Zahoor Khan, Genetic polymorphisms in immune- and inflammationassociated genes and their association with bovine mastitis resistance/susceptibility. [Text] / Muhammad Zahoor Khan// Frontiers in Immunology. TYPE Review Published 23 February 2023 DOI 10.3389/fimmu.2023.1082144
Muhammad Zahoor Khan, Genetic polymorphisms of TRAPPC9 and CD4 genes and their association with milk production and mastitis resistance phenotypic traits in Chinese Holstein. [Text] / Muhammad Zahoor Khan //Frontiers in Veterinary Science. TYPE Original Research PUBLISHED
September 2022 DOI 10.3389/fvets.2022.1008497
Xing Chen, olymorphisms in the selectin gene cluster are associated with fertility and survival time in a population of Holstein Friesian cows. [Text] / Xing Chen// PLoS One. 2017; 12(4): e0175555. doi: 10.1371/journal.pone.0175555
Adrianna Pawlik, Association between interleukin 8 receptor α gene (CXCR1) and mastitis in dairy cattle. [Text] / Adrianna Pawlik// Central European Journal of Immunology 2015; 40(2)
стр 153-158
Pokorska, J. Single nucleotide polymorphisms in the CXCR1 gene and its association with clinical mastitis incidence in Polish Holstein-Friesian cows. [Text] / J. Pokorska // Genetics and Molecular Research 15 (2): gmr.15027247 2016
Masoumeh, B. Estimation of dominance effects related to mastitis and production traits for CXCR1 gene using logistic regression analysis in dairy cattle. [Text] / B. Masoume //Agri Gene Volume 3, March 2017, Pages 63-66
Pushpa Ankit Magotra, Association of CXCR1 gene polymorphism with clinical mastitis, reproductive disorders and performance traits in Hardhenu (Bos taurus × Bos indicus) cattle. [Text] / Pushpa Ankit Magotra// Reprod Domest Anim. 2023 Sep; 58(9):1234-1243.
Hosseini, S.H. Genotyping of Lactoferrin and CXCR1 Genes in Guilan Native Cows and Its Association with Milk Somatic Cell Score. [Text] / S.H. Hosseini Moghaddam, M. Ayatollahi// Iranian Journal of Applied Animal Science (2021) 11(1), 87-93
Ningbo Chen, Genetic Variations of the Bovine MX1 and Their Association with Mastitis. [Text] / Ningbo Chen // Czech J. Anim. Sci., 62, 2017 (4): 157–167
Russell, C.D. Identification of single nucleotide polymorphisms in the bovine toll-like receptor 1 gene and association with health traits in cattle. [Text] / C.D. Russell // Vet. Res., 43: 17.
Awale, M.M. Bovine mastitis: A threat to the economy. [Text] / M.M. Awale // Sci. Rep.,
: 295.
Zhang, C. SLC11A1 gene polymorphisms are not associated to somatic cell score and milk yield in Chinese Holstein. [Text] / C. Zhang // Vet. Immunol. Immunopathol., 127(3-4): 389-292.
Beishova, I. Marking of Genetic Resistance to Chlamydia, Brucellosis and Mastitis in Holstein Cows by Using Polymorphic Variants of LTF, MBL1 and TLR9 Genes. [Text] / Beishova, I. // American Journal of Animal and Veterinary Sciences 2023, 18 (2): 89.97 DOI: 10.3844/ajavsp.2023.89.97
Kawasaki, Y. The effect of single nucleotide polymorphisms in the tumor necrosis factor-α gene on reproductive performance and immune function in dairy cattle [Text] / Y. Kawasaki // J. Reprod. Dev., 2014, vol. 60, no. 3, pp. 173–178. doi 10.1262/jrd.2013-140
Bimenova, Z.Z. Reproductive Function of Cows with Different Genotypes for TNF α Locus and Estimation of Sperm Fertility by the DNA Fragmentation Method. [Text] / Z.Z. Bimenova // Cytology and Genetics, 2019, Vol. 53, No. 1, pp. 42–48. © Allerton Press, Inc., 2019
Zhou, L. Association of novel single nucleotide polymorphisms of the CXCR1 gene with the milk performance traits of Chinese native cattle. [Text] / L. Zhou // Genetics and Molecular Research 12 (3): 2725-2739 (2013)
Beishova, I. Association of polymorphic variants of prolactin (PRL) and beta-lactoglobulin (BLG) genes with resistance/susceptibility to mastitis in holstein cows | Associação de variantes polimórficas dos genes da prolactina (PRL) e betalactoblobulina (BLG) com resistência/suscetibilidade à mastite em vacas holandesas. [Text] / I. Beishova // Brazilian Journal of Biology, 2024, 84, e284961
Beishova, I. Marking of Genetic Resistance to Chlamydia, Brucellosis and Mastitis in Holstein Cows by Using Polymorphic Variants of LTF, MBL1 and TLR9 Genes. [Text] / I. Beishova // American Journal of Animal and Veterinary Sciences, 2023, 18(2), страницы 89–97
Beishova, I.S. Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattle. [Text] / I.S. Beishova // Veterinary World, 2023, 16(1), страницы 161–167
Adrianna Pawlik, Association between interleukin 8 receptor α gene (CXCR1) and mastitis in dairy cattle. [Text] / Adrianna Pawlik //Central European Journal of Immunology 2015; 40(2)
стр 153-158
Pokorska, J. Single nucleotide polymorphisms in the CXCR1 gene and its association with clinical mastitis incidence in Polish Holstein-Friesian cows. [Text] / J. Pokorska // Genetics and Molecular Research 15 (2): gmr.15027247 2016
Zhou, L. Association of novel single nucleotide polymorphisms of the CXCR1 gene with the milk performance traits of Chinese native cattle. [Text] / L. Zhou // Genetics and Molecular Research 12 (3): 2725-2739 (2013)
Leyva-Baca, I. Polymorphisms in the 5′ upstream region of the CXCR1 chemokine receptor gene, and their association with somatic cell score in Holstein cattle in Canada. [Text] / I. Leyva-Baca // J Dairy Sci (2008) 91(1):407–17. doi: 10.3168/jds.2007-0142
Verbeke, J. Somatic cell count and milk neutrophil viability of dairy heifers with specific CXCR1 genotypes following experimental intra-mammary infection with staphylococcus chromogenes originating from milk. [Text] / J. Verbeke //Vet J (2015) 204(3):322–6. doi: 10.1016/ j.tvjl.2015.04.010
REFERENCES
Karthikeyan, A. Genetic basis of mastitis resistance in cattle. International Journal of Science, [Text] / A. Karthikeyan //Environment ISSN 2278-3687 (O) and Technology, Vol. 5, No 4, 2016, 2192 – 2199
Muhammad Zahoor Khan, Genetic polymorphisms in immune- and inflammationassociated genes and their association with bovine mastitis resistance/susceptibility. [Text] / Muhammad Zahoor Khan// Frontiers in Immunology. TYPE Review Published 23 February 2023 DOI 10.3389/fimmu.2023.1082144
Muhammad Zahoor Khan, Genetic polymorphisms of TRAPPC9 and CD4 genes and their association with milk production and mastitis resistance phenotypic traits in Chinese Holstein. [Text] / Muhammad Zahoor Khan //Frontiers in Veterinary Science. TYPE Original Research PUBLISHED
September 2022 DOI 10.3389/fvets.2022.1008497
Xing Chen, olymorphisms in the selectin gene cluster are associated with fertility and survival time in a population of Holstein Friesian cows. [Text] / Xing Chen// PLoS One. 2017; 12(4): e0175555. doi: 10.1371/journal.pone.0175555
Adrianna Pawlik, Association between interleukin 8 receptor α gene (CXCR1) and mastitis in dairy cattle. [Text] / Adrianna Pawlik// Central European Journal of Immunology 2015; 40(2)
str 153-158
Pokorska, J. Single nucleotide polymorphisms in the CXCR1 gene and its association with clinical mastitis incidence in Polish Holstein-Friesian cows. [Text] / J. Pokorska // Genetics and Molecular Research 15 (2): gmr.15027247 2016
Masoumeh, B. Estimation of dominance effects related to mastitis and production traits for CXCR1 gene using logistic regression analysis in dairy cattle. [Text] / B. Masoume //Agri Gene Volume 3, March 2017, Pages 63-66
Pushpa Ankit Magotra, Association of CXCR1 gene polymorphism with clinical mastitis, reproductive disorders and performance traits in Hardhenu (Bos taurus × Bos indicus) cattle. [Text] / Pushpa Ankit Magotra// Reprod Domest Anim. 2023 Sep; 58(9):1234-1243.
Hosseini, S.H. Genotyping of Lactoferrin and CXCR1 Genes in Guilan Native Cows and Its Association with Milk Somatic Cell Score. [Text] / S.H. Hosseini Moghaddam, M. Ayatollahi// Iranian Journal of Applied Animal Science (2021) 11(1), 87-93
Ningbo Chen, Genetic Variations of the Bovine MX1 and Their Association with Mastitis. [Text] / Ningbo Chen // Czech J. Anim. Sci., 62, 2017 (4): 157–167
Russell, C.D. Identification of single nucleotide polymorphisms in the bovine toll-like receptor 1 gene and association with health traits in cattle. [Text] / C.D. Russell // Vet. Res., 43: 17.
Awale, M.M. Bovine mastitis: A threat to the economy. [Text] / M.M. Awale // Sci. Rep.,
: 295.
Zhang, C. SLC11A1 gene polymorphisms are not associated to somatic cell score and milk yield in Chinese Holstein. [Text] / C. Zhang // Vet. Immunol. Immunopathol., 127(3-4): 389-292.
Beishova, I. Marking of Genetic Resistance to Chlamydia, Brucellosis and Mastitis in Holstein Cows by Using Polymorphic Variants of LTF, MBL1 and TLR9 Genes. [Text] / Beishova, I. // American Journal of Animal and Veterinary Sciences 2023, 18 (2): 89.97 DOI: 10.3844/ajavsp.2023.89.97
Kawasaki, Y. The effect of single nucleotide polymorphisms in the tumor necrosis factor-α gene on reproductive performance and immune function in dairy cattle [Text] / Y. Kawasaki // J. Reprod. Dev., 2014, vol. 60, no. 3, pp. 173–178. doi 10.1262/jrd.2013-140
Bimenova, Z.Z. Reproductive Function of Cows with Different Genotypes for TNF α Locus and Estimation of Sperm Fertility by the DNA Fragmentation Method. [Text] / Z.Z. Bimenova // Cytology and Genetics, 2019, Vol. 53, No. 1, pp. 42–48. © Allerton Press, Inc., 2019
Zhou, L. Association of novel single nucleotide polymorphisms of the CXCR1 gene with the milk performance traits of Chinese native cattle. [Text] / L. Zhou // Genetics and Molecular Research 12 (3): 2725-2739 (2013)
Beishova, I. Association of polymorphic variants of prolactin (PRL) and beta-lactoglobulin (BLG) genes with resistance/susceptibility to mastitis in holstein cows | Associação de variantes polimórficas dos genes da prolactina (PRL) e betalactoblobulina (BLG) com resistência/suscetibilidade à mastite em vacas holandesas. [Text] / I. Beishova // Brazilian Journal of Biology, 2024, 84, e284961
Beishova, I. Marking of Genetic Resistance to Chlamydia, Brucellosis and Mastitis in Holstein Cows by Using Polymorphic Variants of LTF, MBL1 and TLR9 Genes. [Text] / I. Beishova // American Journal of Animal and Veterinary Sciences, 2023, 18(2), stranicy 89–97
Beishova, I.S. Genetic polymorphism of prolactin and nitric oxide synthase in Holstein cattle. [Text] / I.S. Beishova // Veterinary World, 2023, 16(1), stranicy 161–167
Adrianna Pawlik, Association between interleukin 8 receptor α gene (CXCR1) and mastitis in dairy cattle. [Text] / Adrianna Pawlik //Central European Journal of Immunology 2015; 40(2) str 153-158
Pokorska, J. Single nucleotide polymorphisms in the CXCR1 gene and its association with clinical mastitis incidence in Polish Holstein-Friesian cows. [Text] / J. Pokorska // Genetics and Molecular Research 15 (2): gmr.15027247 2016
Zhou, L. Association of novel single nucleotide polymorphisms of the CXCR1 gene with the milk performance traits of Chinese native cattle. [Text] / L. Zhou // Genetics and Molecular Research 12 (3): 2725-2739 (2013)
Leyva-Baca, I. Polymorphisms in the 5′ upstream region of the CXCR1 chemokine receptor gene, and their association with somatic cell score in Holstein cattle in Canada. [Text] / I. Leyva-Baca // J Dairy Sci (2008) 91(1):407–17. doi: 10.3168/jds.2007-0142
Verbeke, J. Somatic cell count and milk neutrophil viability of dairy heifers with specific CXCR1 genotypes following experimental intra-mammary infection with staphylococcus chromogenes originating from milk. [Text] / J. Verbeke //Vet J (2015) 204(3):322–6. doi: 10.1016/ j.tvjl.2015.04.010
