ГЕНЕТИЧЕСКИЙ ПОЛИМОРФИЗМ ЭКОПОПУЛЯЦИЙ RHODIOLA LINEARIFOLIA BORISS. КАК МЕХАНИЗМ АДАПТАЦИИ К УСЛОВИЯМ ОКРУЖАЮЩЕЙ СРЕДЫ
ГЕНЕТИЧЕСКИЙ ПОЛИМОРФИЗМ ЭКОПОПУЛЯЦИЙ RHODIOLA LINEARIFOLIA BORISS. КАК МЕХАНИЗМ АДАПТАЦИИ К УСЛОВИЯМ ОКРУЖАЮЩЕЙ СРЕДЫ
DOI:
https://doi.org/10.52578/2305-9397-2025-2-4-138-152Аннотация
Rhodiola linearifolia Boriss. — редкий высокогорный вид рода Rhodiola, обладающий выраженными адаптивными и лекарственными свойствами, однако его генетическая структура до настоящего времени была недостаточно изучена. В данной работе проведён молекулярно-генетический анализ популяций R. linearifolia, произрастающих в условиях Северного Тянь Шаня, с целью оценки их генетического разнообразия и адаптационного потенциала к абиотическим стрессам. Использование метода EPIC-PCR позволило исследовать полиморфизм ключевых генов, вовлечённых в сигнальные пути фитогормона ауксина (ARF6) и антиоксидантной системы (SOD). Установлено, что популяции демонстрируют различную степень генетической изменчивости, а наибольший уровень разнообразия по генам SOD выявлен у популяции BAU. Показано, что вариации в генах ARF и SOD могут играть важную роль в адаптации растений к экстремальным климатическим условиям высокогорий без значительных морфофизиологических изменений. Полученные результаты подчёркивают значимость скрытой генетической изменчивости как основы адаптации редких видов к изменяющимся условиям среды и представляют ценность для дальнейших исследований в области охраны растительного генофонда и устойчивого использования природных ресурсов.
Библиографические ссылки
СПИСОК ЛИТЕРАТУРЫ
Marchev A.S., Dinkova-Kostova A.T., György, Z., Mirmazloum I., Aneva I.Y., Georgiev M.I. Rhodiola rosea L.: From golden root to green cell factories. Phytochem. Rev. 2016. 15. 515–536.
Olfelt, J.P., Freyman W.A. Relationships of north american members of rhodiola (crassulaceae). Botany 2014. 92, 901–910.
Li Y., Pham V., Bui M., Song L., Wu C., Walia A., Uchio E., Smith-Liu F., Zi X. Rhodiola rosea L.: An herb with anti-stress, anti-aging, and immunostimulating properties for cancer chemoprevention. Curr. Pharmacol. Rep. 2017. 3. 384–395.
Pu W.L., Zhang M.Y., Bai R.Y., Sun L.K., Li W.H., Yu Y.L., Zhang Y., Song L., Wang Z.X., Peng Y.F., et al. Anti-inflammatory effects of Rhodiola rosea L.: A review. Biomed. Pharmacother. 2020. 121. 109552.
Dimpfel W., Schombert L., Panossian A.G. Assessing the quality and potential efficacy of commercial extracts of Rhodiola rosea L. By analyzing the salidroside and rosavin content and the electrophysiological activity in hippocampal long-term potentiation, a synaptic model of memory. Front. Pharmacol. 2018. 9. 425.
Tao H., Wu X., Cao J., Peng Y., Wang A., Pei J., Xiao J., Wang S., Wang Y. Rhodiola species: A comprehensive review of traditional use, phytochemistry, pharmacology, toxicity, and clinical study. Med. Res. Rev. 2019. 39. 1779–1850.
Mayuzumi S., Ohba H. The phylogenetic position of eastern asian sedoideae (crassulaceae) inferred from chloroplast and nuclear DNAsequences. Syst. Botany 2004. 29. 587–598.
Ohba H. A revision of the asiatic species of sedoideae crassulaceae 1. Rosularia and rhodiola subgenera primuloides and crassipedes. J. Fac. Sci. Univ. Tokyo 1980. 12. 337–405.
Chaldanbayeva A.K. Biological, Pharmacognostic, and Pharmacological Properties of Rhodiola Linearifolia in Kyrgyzstan.; Saint Petersburg: Saint Petersburg, Russia, June 27 2006. pp. 364–370.
Terletskaya N.V., Erbay M., Mamirova A., Ashimuly K., Korbozova N.K., Zorbekova A.N., Kudrina N.O., Hoffmann M.H. Altitude-Dependent Morphophysiological, Anatomical, and Metabolomic Adaptations in Rhodiola Linearifolia Boriss. Plants 2024. 13. 2698. doi:10.3390/plants13192698.
Sedley L. Advances in nutritional epigenetics-a fresh perspective for an old idea. Lessons learned, limitations, and future directions. Epigenet Insights 2020. 13. 2516865720981924.
Kahlon P.S., Stam, R. Polymorphisms in plants to restrict losses to pathogens: From gene family expansions to complex network evolution. Curr. Opin. Plant Biol. 2021. 62. 102040.
Jain M., Khurana J.P. Transcript profiling reveals diverse roles of auxin-responsive genes during reproductive development and abiotic stress in rice. FEBS J. 2009. 276. 3148–3162.
Song Y., Wang L., Xiong L. Comprehensive expression profiling analysis of osiaa gene family in developmental processes and in response to phytohormone and stress treatments. Planta 2009. 229. 577–591.
Li S.B., Xie Z.Z., Hu C.G., Zhang J.Z. A review of auxin response factors (arfs) in plants. Front. Plant Sci. 2016. 7. 47.
Guilfoyle T.J., Hagen G. Getting a Grasp on Domain III/IV Responsible for Auxin Response Factor–IAA Protein Interactions. Plant Sci. 2012, 190, 82–88.
Kelley D.R., Arreola A., Gallagher T.L., Gasser C.S. ETTIN (ARF3) Physically Interacts with KANADI Proteins to Form a Functional Complex Essential for Integument Development and Polarity Determination in Arabidopsis. Development 2012. 139. 1105–1109.
Mockaitis K., Estelle M. Auxin Receptors and Plant Development: A New Signaling Paradigm. Annu. Rev. Cell Dev. Biol. 2008. 24. 55–80.
Su L., Xu M., Zhang J., Wang Y., Lei Y., Li Q. Genome-Wide Identification of Auxin Response Factor (ARF) Family in Kiwifruit (Actinidia chinensis) and Analysis of Their Inducible Involvements in Abiotic Stresses. Physiol. Mol. Biol. Plants 2021. 27. 1261–1276.
Jain M., Khurana J.P. Transcript Profiling Reveals Diverse Roles of Auxin-Responsive Genes during Reproductive Development and Abiotic Stress in Rice. FEBS J. 2009. 276. 3148–3162.
Li S.-B., Xie Z.-Z., Hu C.-G., Zhang J.-Z. A Review of Auxin Response Factors (ARFs) in Plants. Front. Plant Sci. 2016. 7. 47.
Feng K., Yu J., Cheng Y., Ruan M., Wang R., Ye Q., Zhou G., Li Z., Yao Z., Yang Y., et al. The SOD Gene Family in Tomato: Identification, Phylogenetic Relationships, and Expression Patterns. Front. Plant Sci. 2016. 7. 1279.
Wang J., Li C., Li L., Reynolds M., Mao X., Jing R. Exploitation of Drought Tolerance-Related Genes for Crop Improvement. Int. J. Mol. Sci. 2021. 22. 10265.
Jacobsen S.E., Mujica A., Ortiz R. The Global Potential for Quinoa and Other Andean Crops. Food Rev. Int. 2003. 19. 139–148.
Atkinson N.J., Urwin P.E. The interaction of plant biotic and abiotic stresses: From genes to the field. J. Exp. Bot. 2012. 63. 3523–3543.
Givnish T.J., Evans T.M., Zjhra M.L., Patterson T.B., Berry P.E., Sytsma K.J. Molecular evolution, adaptive radiation, and geographic diversification in the amphiatlantic family rapateaceae: Evidence from ndhf sequences and morphology. Evolution 2000. 54. 1915–1937.
Singh, S.; Singh, P.; Rangabhashiyam, S.; Srivastava, K.K. Global climate change. In Global Climate Change; Elsevier: Amsterdam, The Netherlands, 2021; p. 425.
Deivendran, S. Genetic variability of populations of nilaparvata lugens (stal)(delphacidae: Hemiptera) as revealed by random amplified polymorphic DNA. Biolife 2015, 3, 40–49.
Wani, S.H.; Kumar, V.; Shriram, V.; Sah, S.K. Phytohormones and their metabolic engineering for abiotic stress tolerance in crop plants. Crop J. 2016, 4, 162–176. Genes 2023, 14, 794 14 of 14
Bouzroud, S.; Gouiaa, S.; Hu, N.; Bernadac, A.; Mila, I.; Bendaou, N.; Smouni, A.; Bouzayen, M.; Zouine, M. Auxin response factors (arfs) are potential mediators of auxin action in tomato response to biotic and abiotic stress (Solanum lycopersicum). PLoS ONE2018, 13, e0193517.
Ori, N. Dissecting the biological functions of arf and aux/iaa genes. Plant Cell 2019, 31, 1210–1211.
Duan,Y.-W.; Liu, J.-Q. Pollinator shift and reproductive performance of the qinghai–tibetan plateau endemic and endangered swertia przewalskii (gentianaceae). In Plant Conservation and Biodiversity; Hawksworth, D.L., Bull, A.T., Eds.; Springer: Dordrecht, The Netherlands, 2006; Volume 6, pp. 265–276.
Terletskaya, N.V.; Lee, T.E.; Altayeva, N.A.; Kudrina, N.O.; Blavachinskaya, I.V.; Erezhetova, U. Some mechanisms modulating the root growth of various wheat species under osmotic-stress conditions. Plants 2020, 9, 1545.
Wang,W.; Zhang, X.; Deng, F.; Yuan, R.; Shen, F. Genome-wide characterization and expression analyses of superoxide dismutase (sod) genes in gossypium hirsutum. BMC Genom. 2017, 18, 376.
Zameer, R.; Fatima, K.; Azeem, F.; HIM, A.L.; Sadaqat, M.; Rasheed, A.; Batool, R.; Shah, A.N.; Zaynab, M.; Shah, A.A.; et al. Genome-wide characterization of superoxide dismutase (SOD) genes in daucus carota: Novel insights into structure, expression, and binding interaction with hydrogen peroxide (H2O2) under abiotic stress condition. Front. Plant Sci. 2022, 13, 870241.
Terletskaya, N.V.; Turzhanova, A.S.; Khapilina, O.N.; Zhumagul, M.Z.; Meduntseva, N.D.; Kudrina, N.O.; Korbozova, N.K.; Kubentayev, S.A.; Kalendar, R. Genetic Diversity in Natural Populations of Rhodiola Species of Different Adaptation Strategies. Genes 2023, 14, 794. https://doi.org/ 10.3390/genes14040794
Terletskaya, N.V.; Khapilina, O.N.; Turzhanova, A.S.; Erbay, M.; Magzumova, S.; Mamirova, A. Genetic Polymorphism in the Amaranthaceae Species in the Context of Stress Tolerance. Plants 2023, 12, 3470. https://doi.org/ 10.3390/plants12193470
REFERENCES
Marchev A.S., Dinkova-Kostova A.T., György, Z., Mirmazloum I., Aneva I.Y., Georgiev M.I. Rhodiola rosea L.: From golden root to green cell factories. Phytochem. Rev. 2016. 15. 515–536.
Olfelt, J.P., Freyman W.A. Relationships of north american members of rhodiola (crassulaceae). Botany 2014. 92, 901–910.
Li Y., Pham V., Bui M., Song L., Wu C., Walia A., Uchio E., Smith-Liu F., Zi X. Rhodiola rosea L.: An herb with anti-stress, anti-aging, and immunostimulating properties for cancer chemoprevention. Curr. Pharmacol. Rep. 2017. 3. 384–395.
Pu W.L., Zhang M.Y., Bai R.Y., Sun L.K., Li W.H., Yu Y.L., Zhang Y., Song L., Wang Z.X., Peng Y.F., et al. Anti-inflammatory effects of Rhodiola rosea L.: A review. Biomed. Pharmacother. 2020. 121. 109552.
Dimpfel W., Schombert L., Panossian A.G. Assessing the quality and potential efficacy of commercial extracts of Rhodiola rosea L. By analyzing the salidroside and rosavin content and the electrophysiological activity in hippocampal long-term potentiation, a synaptic model of memory. Front. Pharmacol. 2018. 9. 425.
Tao H., Wu X., Cao J., Peng Y., Wang A., Pei J., Xiao J., Wang S., Wang Y. Rhodiola species: A comprehensive review of traditional use, phytochemistry, pharmacology, toxicity, and clinical study. Med. Res. Rev. 2019. 39. 1779–1850.
Mayuzumi S., Ohba H. The phylogenetic position of eastern asian sedoideae (crassulaceae) inferred from chloroplast and nuclear DNAsequences. Syst. Botany 2004. 29. 587–598.
Ohba H. A revision of the asiatic species of sedoideae crassulaceae 1. Rosularia and rhodiola subgenera primuloides and crassipedes. J. Fac. Sci. Univ. Tokyo 1980. 12. 337–405.
Chaldanbayeva A.K. Biological, Pharmacognostic, and Pharmacological Properties of Rhodiola Linearifolia in Kyrgyzstan.; Saint Petersburg: Saint Petersburg, Russia, June 27 2006. pp. 364–370.
Terletskaya N.V., Erbay M., Mamirova A., Ashimuly K., Korbozova N.K., Zorbekova A.N., Kudrina N.O., Hoffmann M.H. Altitude-Dependent Morphophysiological, Anatomical, and Metabolomic Adaptations in Rhodiola Linearifolia Boriss. Plants 2024. 13. 2698. doi:10.3390/plants13192698.
Sedley L. Advances in nutritional epigenetics-a fresh perspective for an old idea. Lessons learned, limitations, and future directions. Epigenet Insights 2020. 13. 2516865720981924.
Kahlon P.S., Stam, R. Polymorphisms in plants to restrict losses to pathogens: From gene family expansions to complex network evolution. Curr. Opin. Plant Biol. 2021. 62. 102040.
Jain M., Khurana J.P. Transcript profiling reveals diverse roles of auxin-responsive genes during reproductive development and abiotic stress in rice. FEBS J. 2009. 276. 3148–3162.
Song Y., Wang L., Xiong L. Comprehensive expression profiling analysis of osiaa gene family in developmental processes and in response to phytohormone and stress treatments. Planta 2009. 229. 577–591.
Li S.B., Xie Z.Z., Hu C.G., Zhang J.Z. A review of auxin response factors (arfs) in plants. Front. Plant Sci. 2016. 7. 47.
Guilfoyle T.J., Hagen G. Getting a Grasp on Domain III/IV Responsible for Auxin Response Factor–IAA Protein Interactions. Plant Sci. 2012, 190, 82–88.
Kelley D.R., Arreola A., Gallagher T.L., Gasser C.S. ETTIN (ARF3) Physically Interacts with KANADI Proteins to Form a Functional Complex Essential for Integument Development and Polarity Determination in Arabidopsis. Development 2012. 139. 1105–1109.
Mockaitis K., Estelle M. Auxin Receptors and Plant Development: A New Signaling Paradigm. Annu. Rev. Cell Dev. Biol. 2008. 24. 55–80.
Su L., Xu M., Zhang J., Wang Y., Lei Y., Li Q. Genome-Wide Identification of Auxin Response Factor (ARF) Family in Kiwifruit (Actinidia chinensis) and Analysis of Their Inducible Involvements in Abiotic Stresses. Physiol. Mol. Biol. Plants 2021. 27. 1261–1276.
Jain M., Khurana J.P. Transcript Profiling Reveals Diverse Roles of Auxin-Responsive Genes during Reproductive Development and Abiotic Stress in Rice. FEBS J. 2009. 276. 3148–3162.
Li S.-B., Xie Z.-Z., Hu C.-G., Zhang J.-Z. A Review of Auxin Response Factors (ARFs) in Plants. Front. Plant Sci. 2016. 7. 47.
Feng K., Yu J., Cheng Y., Ruan M., Wang R., Ye Q., Zhou G., Li Z., Yao Z., Yang Y., et al. The SOD Gene Family in Tomato: Identification, Phylogenetic Relationships, and Expression Patterns. Front. Plant Sci. 2016. 7. 1279.
Wang J., Li C., Li L., Reynolds M., Mao X., Jing R. Exploitation of Drought Tolerance-Related Genes for Crop Improvement. Int. J. Mol. Sci. 2021. 22. 10265.
Jacobsen S.E., Mujica A., Ortiz R. The Global Potential for Quinoa and Other Andean Crops. Food Rev. Int. 2003. 19. 139–148.
Atkinson N.J., Urwin P.E. The interaction of plant biotic and abiotic stresses: From genes to the field. J. Exp. Bot. 2012. 63. 3523–3543.
Givnish T.J., Evans T.M., Zjhra M.L., Patterson T.B., Berry P.E., Sytsma K.J. Molecular evolution, adaptive radiation, and geographic diversification in the amphiatlantic family rapateaceae: Evidence from ndhf sequences and morphology. Evolution 2000. 54. 1915–1937.
Singh, S.; Singh, P.; Rangabhashiyam, S.; Srivastava, K.K. Global climate change. In Global Climate Change; Elsevier: Amsterdam, The Netherlands, 2021; p. 425.
Deivendran, S. Genetic variability of populations of nilaparvata lugens (stal)(delphacidae: Hemiptera) as revealed by random amplified polymorphic DNA. Biolife 2015, 3, 40–49.
Wani, S.H.; Kumar, V.; Shriram, V.; Sah, S.K. Phytohormones and their metabolic engineering for abiotic stress tolerance in crop plants. Crop J. 2016, 4, 162–176. Genes 2023, 14, 794 14 of 14
Bouzroud, S.; Gouiaa, S.; Hu, N.; Bernadac, A.; Mila, I.; Bendaou, N.; Smouni, A.; Bouzayen, M.; Zouine, M. Auxin response factors (arfs) are potential mediators of auxin action in tomato response to biotic and abiotic stress (Solanum lycopersicum). PLoS ONE2018, 13, e0193517.
Ori, N. Dissecting the biological functions of arf and aux/iaa genes. Plant Cell 2019, 31, 1210–1211.
Duan,Y.-W.; Liu, J.-Q. Pollinator shift and reproductive performance of the qinghai–tibetan plateau endemic and endangered swertia przewalskii (gentianaceae). In Plant Conservation and Biodiversity; Hawksworth, D.L., Bull, A.T., Eds.; Springer: Dordrecht, The Netherlands, 2006; Volume 6, pp. 265–276.
Terletskaya, N.V.; Lee, T.E.; Altayeva, N.A.; Kudrina, N.O.; Blavachinskaya, I.V.; Erezhetova, U. Some mechanisms modulating the root growth of various wheat species under osmotic-stress conditions. Plants 2020, 9, 1545.
Wang,W.; Zhang, X.; Deng, F.; Yuan, R.; Shen, F. Genome-wide characterization and expression analyses of superoxide dismutase (sod) genes in gossypium hirsutum. BMC Genom. 2017, 18, 376.
Zameer, R.; Fatima, K.; Azeem, F.; HIM, A.L.; Sadaqat, M.; Rasheed, A.; Batool, R.; Shah, A.N.; Zaynab, M.; Shah, A.A.; et al. Genome-wide characterization of superoxide dismutase (SOD) genes in daucus carota: Novel insights into structure, expression, and binding interaction with hydrogen peroxide (H2O2) under abiotic stress condition. Front. Plant Sci. 2022, 13, 870241.
Terletskaya, N.V.; Turzhanova, A.S.; Khapilina, O.N.; Zhumagul, M.Z.; Meduntseva, N.D.; Kudrina, N.O.; Korbozova, N.K.; Kubentayev, S.A.; Kalendar, R. Genetic Diversity in Natural Populations of Rhodiola Species of Different Adaptation Strategies. Genes 2023, 14, 794. https://doi.org/ 10.3390/genes14040794
Terletskaya, N.V.; Khapilina, O.N.; Turzhanova, A.S.; Erbay, M.; Magzumova, S.; Mamirova, A. Genetic Polymorphism in the Amaranthaceae Species in the Context of Stress Tolerance. Plants 2023, 12, 3470. https://doi.org/ 10.3390/plants12193470
