GENETIC DIVERSITY OF THE KOSTANAY HORSE BREED USING MICROSATELLITE MARKERS
DOI:
https://doi.org/10.52578/2305-9397-2023-2-19-26Ключевые слова:
horses, Kostanay breed, microsatellites, locus, DNA.Аннотация
The article presents the results of the analysis of genetic diversity of the Kostanay breed of 17 loci of DNA microsatellites, including AHT4, AHT5, ASB17, ASB2, ASB23, CA425, HMS1, HMS2, HMS3, HMS6, HMS7, HTG10, HTG4, HTG6, HTG7, LEX3, VHL20. Hair bulbs of 30 heads of horses of Kostanay breed (Kostanay city, Zarechny settlement) were used as biological material for research.
The aim of the work was to characterise the genetic diversity of horses of the Kostanay breed by microsatellite STR-loci.
Genotyping of animals was carried out by PCR, separation and detection of amplification products was carried out by capillary electrophoresis on an 8-capillary 3500 Genetic Analyzer (Applied Biosystems, USA). The results were interpreted in GeneMapper programme, and statistical processing was performed using Microsoft Excel 2010. The following parameters were calculated: allele frequencies, polymorphism level (Ae), degree of observed (Ho) and expected (Не) heterozygosity, fixation index (Fis).
As a result of genotyping of the Kostanay breed of horses bred in Zarechny settlement of Kostanay district on the studied microsatellite loci, data characterising the polymorphism of each of the markers were obtained. On 17 microsatellite loci of DNA in horses of Kostanay breed 102 alleles were revealed. The number of alleles per locus varied from 4 to 10. The most informative for the group under study were loci with the highest number of alleles (ASB17, ASB2), the least informative - with the minimum (HMS1, HTG7). The highest observed heterozygosity was at the ASB2 locus (0.900) and the lowest at the HMS2 locus (0.367). The average expected heterozygosity was 0.696, observed heterozygosity was 0.681, and the mean fixation index had a value equal to 0.018.
Thus, characteristic features of polymorphism on all investigated indexes of the studied microsatellite loci of DNA of the Kostanay breed of horses were revealed.
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