VETERINARY SANITARY EXAMINATION OF FISH WHEN USING SYMBIOTICS
DOI:
https://doi.org/10.52578/2305-9397-2025-3-1-13-21%20Ключевые слова:
African catfish, symbiotics, microbiome, aquaculture, veterinary-sanitary examinationАннотация
The present study investigates the effect of symbiotic feed additives on the veterinary sanitary quality of African catfish (Clarias gariepinus) raised under aquaculture conditions in southern Kazakhstan. Symbiotics - combinations of probiotics and prebiotics—have emerged as promising alternatives to antibiotics in modern aquaculture due to their ability to modulate gut microbiota, enhance nutrient absorption, and improve immune response.
In this experiment, catfish were divided into control and experimental groups, with the latter receiving a complex symbiotic supplement containing lactic acid bacteria and enzymatic components. Over a 28-day feeding period, parameters such as growth rate, organoleptic characteristics, physicochemical properties (pH, peroxidase activity), and biochemical freshness indicators (ammoniacal nitrogen) were assessed.
The results showed a significant improvement in growth performance (final weight and feed conversion ratio), enhanced muscle texture and appearance, and positive peroxidase and pH readings indicating high product freshness. Organoleptic analysis revealed superior external quality and tissue integrity in the experimental group. Furthermore, the absence of spoilage indicators confirmed microbiological safety of the fish meat.
This study demonstrates that the use of symbiotics in aquaculture not only improves production efficiency but also contributes to the sanitary safety and sensory quality of fish products. Veterinary sanitary examination methods proved essential in confirming these effects. The findings support the integration of microbiome-based dietary strategies as a sustainable approach in fish farming systems and underscore the importance of sanitary control in protecting public health.
Библиографические ссылки
Sullam, K. E., Essinger, S. D., Lozupone, C. A., O’Connor, M. P., Rosen, G. L., Knight, R., Kilham, S. S., Russell, J. A. Environmental and ecological factors that shape the gut bacterial communities of fish: A meta-analysis [Text] / K. E. Sullam [and etc.] // Molecular Ecology. – 2012. – Vol. 21, No. 13. – P. 3363–3378. https://doi.org/10.1111/j.1365-294X.2012.05552.x.
ICES (International Council for the Exploration of the Sea). ICES Annual Report 2011 [Text] / ICES. – Copenhagen, Denmark. – 2011. https://www.ices.dk.
Bøgwald, J., Dalmo, R. A. Review on immune responses in salmonid fish [Text] / J. Bøgwald [and etc.] // Journal of Fish Biology. – 2014. – Vol. 85, No. 5. – P. 1447–1471. https://doi.org/10.1111/jfb.12504.
Merrifield, D. L., Rodiles, A. The fish microbiome and its interactions with mucosal tissues [Text] / D. L. Merrifield [and etc.] // In: Mucosal Health in Aquaculture / Eds. M. Beck, E. Peatman. – Academic Press, 2015. – P. 273–295. https://doi.org/10.1016/B978-0-12-417186-2.00011-4.
Gomez, G. D., Balcázar, J. L. A review on the interactions between gut microbiota and innate immunity of fish [Text] / G. D. Gomez [and etc.] // FEMS Immunology & Medical Microbiology. – 2008. – Vol. 52, No. 2. – P. 145–154. https://doi.org/10.1111/j.1574-695X.2007.00343.x.
Egerton, S., Culloty, S., Whooley, J., Stanton, C., Ross, R. P. The gut microbiota of marine fish [Text] / S. Egerton [and etc.] // Frontiers in Microbiology. – 2018. – Vol. 9. – P. 873. https://doi.org/10.3389/fmicb.2018.00873.
Sylvain, F. É., Cheaib, B., Llewellyn, M., Correia, T. G., Fagundes, D. B., Val, A. L., Derome, N. pH drop impacts differentially skin and gut microbiota of the Amazonian fish tambaqui (Colossoma macropomum) [Text] / F. É. Sylvain [and etc.] // Scientific Reports. – 2016. – Vol. 6. – P. 32032. https://doi.org/10.1038/srep32032.
Tarnecki, A. M., Burgos, F. A., Ray, C. L., Arias, C. R. Fish intestinal microbiome: Diversity and symbiosis unravelled by metagenomics [Text] / A. M. Tarnecki [and etc.] // Journal of Applied Microbiology. – 2017. – Vol. 123, No. 1. – P. 2–17. https://doi.org/10.1111/jam.13415.
Borges, N., Keller-Costa, T., Silva, S. G., Gonçalves, J. F. M., Costa, R. Probiotics and prebiotics as functional feed ingredients for aquaculture: Benefits on fish health and sustainability [Text] / N. Borges [and etc.] // Aquaculture Reports. – 2021. – Vol. 20. – P. 100700. https://doi.org/10.1016/j.aqrep.2021.100700.
de Bruijn, I., Liu, Y., Wiegertjes, G. F., Raaijmakers, J. M. Exploring fish microbial communities to mitigate emerging diseases in aquaculture [Text] / I. de Bruijn [and etc.] // FEMS Microbiology Ecology. – 2017. – Vol. 94, No. 1. – P. fix161. https://doi.org/10.1093/femsec/fix161.
Hai, N. V. The use of probiotics in aquaculture [Text] / N. V. Hai // Journal of Applied Microbiology. – 2015. – Vol. 119, No. 4. – P. 917–935. https://doi.org/10.1111/jam.12886.
Verschuere, L., Rombaut, G., Sorgeloos, P., Verstraete, W. Probiotic bacteria as biological control agents in aquaculture [Text] / L. Verschuere [and etc.] // Microbiology and Molecular Biology Reviews. – 2000. – Vol. 64, No. 4. – P. 655–671. https://doi.org/10.1128/MMBR.64.4.655-671.2000.
Zhou, Z., Zhang, M., Wang, J., He, S. The role and potential of probiotics in aquaculture [Text] / Z. Zhou [and etc.] // Animal Nutrition. – 2014. – Vol. 1, No. 1. – P. 47–50. https://doi.org/10.1016/j.aninu.2015.05.005.
Brandt, K. K., Sørensen, S. J., Burmølle, M. Natural transformation of Acinetobacter baylyi in soil: The role of nutrient availability and bacterial metabolic activity [Text] / K. K. Brandt [and etc.] // FEMS Microbiology Ecology. – 2015. – Vol. 91, No. 7. – P. fiv043. https://doi.org/10.1093/femsec/fiv043.
WHO (World Health Organization). Global action plan on antimicrobial resistance [Text] / World Health Organization. – Geneva. – 2015. https://www.who.int/publications/i/item/9789241509763.
Ringo, E., Olsen, R. E., Jensen, I., Romero, J., Lauzon, H. L. Application of vaccines and dietary supplements in aquaculture: Possibilities and challenges [Text] / E. Ringo [and etc.] // Reviews in Fish Biology and Fisheries. – 2010. – Vol. 20, No. 1. – P. 1–22. https://doi.org/10.1007/s11160-009-9124-3.
Pandiyan, P., Balaraman, D., Thirunavukkarasu, R., George, E. G. J., Subaramaniyan, K., Manikkam, S., Sadayappan, B. Probiotics in aquaculture [Text] / P. Pandiyan [and etc.] // Drug Invention Today. – 2013. – Vol. 5, No. 1. – P. 55–59. https://doi.org/10.1016/j.dit.2013.03.003.
Guerreiro, I., Magalhães, R., Coutinho, F., Couto, A., Serra, C. R., Enes, P., Oliva-Teles, A. Effect of dietary inulin and mannan oligosaccharides on growth, intestinal morphology and gut microbiota of European sea bass (Dicentrarchus labrax) juveniles [Text] / I. Guerreiro [and etc.] // Aquaculture Nutrition. – 2018. – Vol. 24, No. 2. – P. 465–475. https://doi.org/10.1111/anu.12583.
Zhao, J., Song, Y., Zhuang, P., Zhang, L., Wang, Y., Yang, G. Effects of synbiotics on growth performance, serum immune responses, antioxidant status and gut microbiota of juvenile largemouth bass (Micropterus salmoides) [Text] / J. Zhao [and etc.] // Aquaculture. – 2020. – Vol. 528. – Article ID 735509. https://doi.org/10.1016/j.aquaculture.2020.735509.
Dawood, M. A. O., Koshio, S., Esteban, M. Á. Beneficial roles of feed additives as immunostimulants in aquaculture: a review [Text] / M. A. O. Dawood [and etc.] // Reviews in Aquaculture. – 2018. – Vol. 10, No. 4. – P. 950–974.
Beveridge, T. J. Use of the Gram stain in microbiology [Text] / T. J. Beveridge // Biotechnic & Histochemistry. – 2001. – Vol. 76, No. 3. – P. 111–118. https://doi.org/10.3109/10520290109045278.
Hoseinifar, S. H., et al. The effects of prebiotics on immune response and performance of fish [Text] / S. H. Hoseinifar [and etc.] // Aquaculture. – 2017. – Vol. 473. – P. 379–384. https://doi.org/10.1016/j.aquaculture.2017.06.006.
Dawood, M. A. O., Koshio, S. Recent advances in the role of probiotics and prebiotics in carp aquaculture [Text] / M. A. O. Dawood [and etc.] // Fish & Shellfish Immunology. – 2019. – Vol. 92. – P. 209–218. https://doi.org/10.1016/j.fsi.2018.11.034.
Zhang, J., et al. Synergistic effect of probiotics and prebiotics on enhancing microbial safety and quality of aquaculture products [Text] / J. Zhang [and etc.] // Applied Microbiology and Biotechnology. – 2012. – Vol. 94. – P. 1021–1027. https://doi.org/10.1007/s00253-011-3432-3.
Skroza, D., et al. Influence of feeding strategies on fish quality parameters [Text] / D. Skroza [and etc.] // LWT – Food Science and Technology. – 2021. – Vol. 146. – Article ID 110919. https://doi.org/10.1016/j.lwt.2021.110919.
Mohammadi, G., et al. Application of natural antioxidants in fish preservation: A review [Text] / G. Mohammadi [and etc.] // LWT – Food Science and Technology. – 2020. – Vol. 113. – Article ID 108502. https://doi.org/10.1016/j.lwt.2019.108502.
Martins, C. I. M., et al. Hygiene and parasite management in recirculating aquaculture systems [Text] / C. I. M. Martins [and etc.] // Aquacultural Engineering. – 2020. – Vol. 89. – Article ID 102079. https://doi.org/10.1016/j.aquaeng.2020.102079.