EXAMINATION OF SIGNIFICANT FACTORS AFFECTING THE PROPAGATION OF A SPECIFIC STRAIN OF LUMPY SKIN DISEASE VIRUS IN BOVINE POPULATIONS
AS
DOI:
https://doi.org/10.52578/2305-9397-2025-4-1-192-201Ключевые слова:
warty dermatitis virus, cellular proliferation, growth parameters, infection dosage, temperatureАннотация
The current manuscript delineates the dissemination of the warty dermatitis virus variant "Dermatitis nodularis bovum/2018/Kostanay/KZ" through cell lines derived from ovine kidney, ovine testis, bovine embryonic kidney (MDBK), Syrian infant monkey kidney (VNK-21), and African green monkey kidney (Vero), while concurrently presenting the outcomes of an inquiry aimed at determining the optimal conditions for virus cultivation within the designated cell culture of this particular strain. According to the results of the inquiry, it was ascertained that, among the assessed cell cultures, those originating from ovine kidney and ovine testis displayed a marked susceptibility to the viral proliferation. The pivotal parameters affecting viral growth within the specified cell culture were identified, including the infectious dose – varying from 0.1 to 0.001 TCD50/cell; the optimal incubation temperature – sustained at 36.5°C; serum concentration – established at 2%; and the incubation duration – extending from 96 to 144 hours. In light of these identified efficacious cultivation parameters, the infectivity of the viral strain cultivated in cell culture demonstrated a spectrum ranging from 106.10-6.25 lg TCD50/cm3.Furthermore, during the process of exposing the virus to ten consecutive passages within trypsinized primary cell cultures, the virus demonstrated adaptation to the cellular milieu, culminating in cytopathological modifications within the cells, thereby facilitating the attainment of maximal viral yield. The conclusions derived from this investigation possess substantial implications for prospective applications in the development of prophylactic and diagnostic interventions targeting the warty dermatitis virus.
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