IMMUNOBIOLOGICAL EVALUATION OF THE VIRAL VACCINE AGAINST EQUINE RHINOPNEUMONITIS
DOI:
https://doi.org/10.52578/2305-9397-2025-4-2-16-23Ключевые слова:
Equine rhinopneumonitis, strain, herpesvirus, vaccine, preventionАннотация
During the conducted studies, a dry viral vaccine against equine rhinopneumonitis, “RinoVac”, was developed and experimentally tested. The vaccine was produced using the equine rhinopneumonitis virus strain “AK-2011” on a monolayer culture of TT cells. A comprehensive set of biological tests was carried out, including the evaluation of immunogenicity (CFT, ELISA) and protective activity in laboratory animals, as well as safety assessment in white mice. In laboratory conditions, the procedures for obtaining virus-containing biomass were optimized, and the optimal doses and routes of administration were determined, along with the assessment of immunogenicity – all studies were performed on model rabbits (subcutaneous and intramuscular administration at doses of 0.5, 1.0, and 2.0 cm³). Additionally, the effect of revaccination on the intensity and durability of the immune response was evaluated.
In vaccinated animals, a significant increase in the level of specific antibodies and the formation of a pronounced protective response compared to the control group were recorded. In rabbits, antibody titers reached 1:300 on day 7, increased to 1:500 on day 14, and remained at 1:400 by day 30; the protective effectiveness was 80% (versus 20% in the control group). Revaccination performed 90 days after the primary immunization led to an additional enhancement of the humoral response: in all dilutions (1:50–1:400), optical density values in ELISA in the revaccinated animals were significantly higher than in the control group (for example, at 1:50 – 1.89 vs. 0.73), indicating the development of a more stable and long-lasting immunity.
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